[BioC] FrozenRMA how to
Alejandro Sánchez Pla
sanplaale at gmail.com
Sat Mar 3 18:34:41 CET 2012
Hello
I have a doubt about how to use frma.
I have 30 hgu133plus2 arrays where I would like to find the
"unexpressed" genes. If I have understood it well the "barcode"
function in the frma package can be used to find such genes, or, say
provide a proposal.
The arrays have been obtained in two different batches so frma seems
even more approprite because ir claims to have the ability to process
data from different batches.
My question is then how should I proceed.
My idea is
- Process every batch (two in this case) separately
- Combine the data inti a single object
- Use barcode on this object to call the unexpressed genes.
Can someone confurm me If this is the right approach?
thanks for the help
Alex Sanchez
Statistics Department. Universitar de Barcelona &
Statistics and Bioinformatics Unit. Vall d'Hebron Research Institute
Enviado desde mi iPhone
El 03/03/2012, a las 12:00, <bioconductor-request at r-project.org> escribió:
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> Today's Topics:
>
> 1. Provide an Example for AnnotatedDataFrame (Deepak Datta)
> 2. Re: Difficulty in AnnotatedDataFrame (James F. Reid)
> 3. Re: easyRNASeq error (Nicolas Delhomme)
> 4. Biobase typo in example of AnnotatedDataFrame man page
> (James F. Reid)
> 5. Re: Biobase typo in example of AnnotatedDataFrame man page
> (Martin Morgan)
> 6. Re: easyRNASeq error (Nicolas Delhomme)
> 7. Re: A problem about trimLRPatterns (wang peter)
> 8. Summarizing Single-channel Agilent data (David Westergaard)
> 9. Gene-Metabolite correlation network (pankaj borah)
> 10. Re: problem creating own org.Ss.eg.db (Hooiveld, Guido)
> 11. Re: Summarizing Single-channel Agilent data
> (axel.klenk at actelion.com)
> 12. Re: Gene-Metabolite correlation network (Sean Davis)
> 13. Re: Gene-Metabolite correlation network (pankaj borah)
> 14. Re: A problem about trimLRPatterns (Harris A. Jaffee)
> 15. KEGGSOAP (Ricardo Silva)
> 16. GAGE heatmap pdf question... (Alan Derr)
> 17. Re: Finding coding SNPs with predictCoding (Valerie Obenchain)
> 18. Re: Finding coding SNPs with predictCoding (Thomas Girke)
> 19. Re: KEGGSOAP (Martin Morgan)
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> 22. beadarray vignette has broken code in R2.14.1? (Henriquez, Nick)
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>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 2 Mar 2012 16:29:54 +0530
> From: Deepak Datta <deepaktheinvincible at gmail.com>
> To: bioconductor at r-project.org
> Subject: [BioC] Provide an Example for AnnotatedDataFrame
> Message-ID:
> <CAHzX1SmQ_WUjujhithkt8FHN9cQ1wvFDJCuyFy9h9uYY4=sHRA at mail.gmail.com>
> Content-Type: text/plain
>
> hii... this is Deepak....
>
> i am working on analysis of microarray data using bioconductor and i am
> rank amateur in R
>
> I tried using this code snippet
> phenoData(spikein95) <- new("phenoData", pData = pd, varLabels = vl)
>
> but it said that phenoData is now defunct and we need to use
> AnnotatedDataFrame. And i am not able to proceed because of the following
> error
>
> AnnotatedDataFrame(x1)<-new("AnnotatedDataFrame",pData=pd, varLabels=v1)
> Error in .nextMethod(.Object, ...) :
> invalid names for slots of class ?AnnotatedDataFrame?: pData, varLabels
>
>
> i am basically trying to segregate the samples into 3 parts containing
> normal, HSIL and SCC data and this is the code that i have used till now...
>
> setwd("E:\\Project\\CEL files\\Old\\GSE7803-3")
>> f1=list.celfiles(path="E:\\Project\\CEL files\\Old\\GSE7803-3")
>> x1<-ReadAffy(filenames=f1)
>> pd <- data.frame(population = c(1,1,1,1,1,1,1,1,1,1,2, 2,
> + 2,2,2,2,2,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3,3),
> + replicate= c(1,2,3,4,5,6,7,8,9,10,1,2,3,4,5,6,7,
> + 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21))
>> rownames(pd) <- sampleNames(x1)
>> v1 <- list(population = "1 is normal, 2 is HSIL, 3 is SCC",
> replicate="arbitary numbering")
>
>
> i further wish to perform rma and then generate a MA plot.
>
> kindly help me rectify this error and please explain with an *example* so
> that i could proceed further.
>
> [[alternative HTML version deleted]]
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 02 Mar 2012 11:03:37 +0000
> From: "James F. Reid" <james.reid at ifom-ieo-campus.it>
> To: Deepak Datta <deepaktheinvincible at gmail.com>
> Cc: bioconductor at r-project.org
> Subject: Re: [BioC] Difficulty in AnnotatedDataFrame
> Message-ID: <4F50A909.1020602 at ifom-ieo-campus.it>
> Content-Type: text/plain; charset=windows-1252; format=flowed
>
> Hi Deepak,
>
> please keep the thread on the list for other users.
>
> On 02/03/12 10:28, Deepak Datta wrote:
>> hey James, i have read the help page and i did not find it helpful. i
>> would really appreciate if you could please explain me with an example
>> giving details on creating a metaData
>
>
> something like this should work:
>
> new("AnnotatedDataFrame",
> data=pd,
> varMetadata=data.frame(labelDescription=unlist(v1)))
>
> since your v1 is a list (it needen't be).
>
> Best.
> J.
>
>>
>>
>> On Fri, Mar 2, 2012 at 3:49 PM, James F. Reid
>> <james.reid at ifom-ieo-campus.it <mailto:james.reid at ifom-ieo-campus.it>>
>> wrote:
>>
>> Hi Deepak,
>>
>> you need to construct an AnnotatedDataFrame in the following way:
>> AnnotatedDataFrame(data=df, varMetaData=metaData)
>> Look-up the help page which is well documented:
>> help("AnnotatedDataFrame")
>>
>> HTH.
>> J.
>>
>>
>> On 02/03/12 10:11, Deepak Datta wrote:
>>
>> hii... this is Deepak....
>>
>> i am working on analysis of microarray data.
>>
>> We tried using this code snippet
>> phenoData(spikein95)<- new("phenoData", pData = pd, varLabels = vl)
>>
>> but it said that phenoData is now defunct and we need to use
>> AnnotatedDataFrame. And i am not able to proceed because of the
>> following
>> error
>>
>> AnnotatedDataFrame(x1)<-new("__AnnotatedDataFrame",pData=pd,
>> varLabels=v1)
>> Error in .nextMethod(.Object, ...) :
>> invalid names for slots of class ?AnnotatedDataFrame?: pData,
>> varLabels
>>
>>
>> i am basically trying to segregate the samples into 3 parts
>> containing
>> normal, HSIL and SCC data and this is the code that i have used
>> till now...
>>
>> setwd("E:\\Project\\CEL files\\Old\\GSE7803-3")
>>
>> f1=list.celfiles(path="E:\\__Project\\CEL
>> files\\Old\\GSE7803-3")
>> x1<-ReadAffy(filenames=f1)
>> pd<- data.frame(population = c(1,1,1,1,1,1,1,1,1,1,2, 2,
>>
>> + 2,2,2,2,2,3,3,3,3,3,3,3,3,3,3,__3,3,3,3,3,3,3,3,3,3,3),
>> + replicate= c(1,2,3,4,5,6,7,8,9,10,1,2,3,__4,5,6,7,
>> + 1,2,3,4,5,6,7,8,9,10,11,12,13,__14,15,16,17,18,19,20,21))
>>
>> rownames(pd)<- sampleNames(x1)
>> v1<- list(population = "1 is normal, 2 is HSIL, 3 is SCC",
>>
>> replicate="arbitary numbering")
>>
>>
>> i further wish to perform rma and then generate a MA plot.
>>
>> kindly help me rectify this error and if possible explain with
>> an example
>> so that i could proceed further.
>>
>> [[alternative HTML version deleted]]
>>
>>
>>
>>
>> _________________________________________________
>> Bioconductor mailing list
>> Bioconductor at r-project.org <mailto:Bioconductor at r-project.org>
>> https://stat.ethz.ch/mailman/__listinfo/bioconductor
>> <https://stat.ethz.ch/mailman/listinfo/bioconductor>
>> Search the archives:
>> http://news.gmane.org/gmane.__science.biology.informatics.__conductor
>> <http://news.gmane.org/gmane.science.biology.informatics.conductor>
>>
>>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 2 Mar 2012 12:20:36 +0100
> From: Nicolas Delhomme <delhomme at embl.de>
> To: "Davis, Wade" <davisjwa at health.missouri.edu>
> Cc: Bioconductor mailing list <bioconductor at r-project.org>
> Subject: Re: [BioC] easyRNASeq error
> Message-ID: <8395D3FF-9D14-49E1-855F-E578A39E3089 at embl.de>
> Content-Type: text/plain; charset=us-ascii
>
> Hi Wade,
>
> I'll send you the package off-list. And discuss how we can do for the data as it's still interesting for me to enhance my chromosome name validity checks.
>
> Cheers,
>
> Nico
>
> ---------------------------------------------------------------
> Nicolas Delhomme
>
> Genome Biology Computational Support
>
> European Molecular Biology Laboratory
>
> Tel: +49 6221 387 8310
> Email: nicolas.delhomme at embl.de
> Meyerhofstrasse 1 - Postfach 10.2209
> 69102 Heidelberg, Germany
> ---------------------------------------------------------------
>
>
>
> On 1 Mar 2012, at 23:14, Davis, Wade wrote:
>
>> Hi Nico,
>> If you could send me a windows zip that I could try out the changes now instead of waiting for them to get pushed through in a few days. But I don't mind waiting, whatever is easiest for you.
>>
>> I've been in a workshop all days, so I haven't gotten a chance to try out the suggestions for the second problem, but I will do so and get back to do.
>>
>> Also, you asked for a sample file to help with the second problem. I'm not sure if you still want or need that, but below is the first 60 lines or so. I can send you an entire file (compressed which is about 455MB) if you think that would be helpful.
>>
>> Thanks!
>>
>> Wade
>>
>> HWI-ST538 160 7 1101 1635 1954 ACAGTG 1 GATATCTNNNNNNGAAGAAAACGAGGGAGATTCCAAGGCCGAGCAGCTACACCNGGTACAGAAGCAGTCTGCCACTTTGCCCTCGCCCATCACCCACTCC
>> BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 1661 1972 ACAGTG 1 GGATAGCATNNNAAATGTAAATGAGGAAAATACCTAATAAAAAATATTAAAAAAAAAAAAGACACCGCCAGGAACTTGTGAGAAATCCAAATTCTACATT
>> [[[______BBBQQX_^^^^_^___^]^_^^^^[^^^^^^]^^^^^^^^^^^^^^^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 1949 1944 ACAGTG 1 AGGGGTTNNNNNNTTCAAGACCTCTCCCCTGNNGAGAAAGAAACCCAAGCAGNNGCCTGTGGGAGGGAACACGGACCTGACACCTCTGCCTCAGACTCCT
>> [[XZY_^BBBBBBQQ[\Z\]_[[Z^^]^^[^BBOL[\X^^PY]ZX\^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 1820 1948 ACAGTG 1 GTCAGCTNNNNNNAGGGTCCGATCGTATTGGANGAGGATATCTTTGATCTCCNNCTTGGAGGCTTCATCCACATATTTTTGGTAATAAGCTACCAGGGCC
>> [[[^___BBBBBBRS]_]^__^__^^^^^^^^BO[\^^^^^^^^^\^^^^^]BBLW\\^]_^X^^]\]]]]^^]]]]]^\[VZZ\\\\Y[\\\[\[[Y[[ QC N
>> HWI-ST538 160 7 1101 1773 1957 ACAGTG 1 CTTTGGTTNNNNNGACCTCGCTTGTAGCCAGCAAAAATGGCCTTGCACCACAGCCTTCCAGACATACTTGCTGTTAGAAGTCCTGAGATCGGAAGAGCAC
>> WZZ_[\]_BBBBBRQY_^^\Z^__^^^^^^]^]^]^^^^^^]^^^]]X]]]^^^^^^^___[^^]]^^^]^^W\\]]]\]V\\\][[[\\[[Z[Y[[WZW QC N
>> HWI-ST538 160 7 1101 1971 1962 ACAGTG 1 CTTTGTTTNNNNTCTGGTCACAATAGTTGGGGGCAGAAAAGACAGTGACACAGCGGCCACCATGGGCCACCTCGTAGCCCTCGGCTTTGACTTCATGGCT
>> [[[_____BBBBRR_____^__^________^^^^^^^^^^^^^^^^^^^^^^[^^\\[[^[[[\[[[[[[[[[W[[[\\[[WY[[U[YT[[\[YZSYW[ QC N
>> HWI-ST538 160 7 1101 1878 1976 ACAGTG 1 CCGCTGTCTTNCTCAGGAAGCACGCATTTCCACAGGCCATAGGGCTTTCCTACCACAGTTTGCCACAATCTCAATGTCCCATCTTCAGAACCGCTGGCAT
>> ^W^\cc\`^`BQQ``JY[YRb`^_dUccaSccaIYHOWOXRacQaaQabd\bbcbbcc\^bccc]Z^c_]R_````UZZ]`Y\ZT]]`BBBBBBBBBBBB RM N
>> HWI-ST538 160 7 1101 1934 1999 ACAGTG 1 CTCCACTGAAGTACTTCAGGTAGTAGTCCATGACGCCCTTGTCGTCGGTTCGCGACTCCTCATCACTGTCCCCGGCCCGGCCCACCGATGACATCATCTG
>> bbbeeeeegggeghiiiiiigghgfiiiiihihiihiihiihhfghdgZeefhadeecdbdb`bccbb`bb`aQX[acX[[acO[aOEHOGYRY`]Y`R] chr10.fa 80607676 R 100 500 Y
>> HWI-ST538 160 7 1101 2013 1948 ACAGTG 1 CACATCTNNNNNNCCATGGAGCAGATTGAAGANGAAATCAAAGGTTGTTTGGNNTTTCTTCGCACAGTATATAGTGTCTTTGGATTTTCATTTAAATTGA
>> [[[___^BBBBBBRSX_____^_^_^_^__^^BP[^^^^^^^^^\^^^^^^[BBLW\^^__]^^^^^Z\]]]]^\Z\]]^^][[\\\\\\\\\\\\[\Y[ QC N
>> HWI-ST538 160 7 1101 2064 1957 ACAGTG 1 AGAAAGTTNNNNNATAGAAGATGTATAAGTTGATCGTAACGGAAGCGTGGATAAGATGCTCGGATCCATAGGAATGTTGATGATAGTAGTAGAGCTTCTA
>> [[[[^[\_BBBBBQQ\[\^]__^[_]_^^^_^^__^[^^[[W^^[^^\^^\^^^]^^^___[^[XW]Z]^^BBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 2129 1991 ACAGTG 1 GTAATATTCATTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACTTTGCCAGCTCCAGCAGCCTTCTTGTCCACAGCTTTGATGACACCCACAGCAAC
>> ___ce`cce`ggfdhfhfgbfbefdghggfd`ff]eZegg]eIYYYceg^cedfce^ebgfce_ccfhdfdZbdbV`db]Za^ZaZ]__bbaa_PTGT^` RM Y
>> HWI-ST538 160 7 1101 2267 1959 ACAGTG 1 CAGCACAGNNNNGATGGCTACGTACATGGCTGGGGTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTTTCACGGTTGGCCTTAGGGTTCAGGGGGGCC
>> [[[__^^^BBBBQQ_\^[^]^^^^^[^_^[^^^^^\Z\^RU\]]UY]WXZ^]Z^]Z]^_^^]ZZV^BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 2466 1999 ACAGTG 1 CGTACTTCTCCCACTCGCTTGTGCTCTGTCACTAGAAGGTTATTCTTGAAAGGTTCTGTGCCTTTTTCTTCAGGCTTCATCTGTCATACCTCTACTTGAT
>> bbaeeeeegggggiiifiiiihiiiiihhhiiihighiiafgfhiiifaehghXcghhhhbghiiiifhhfdghhgfedgeddcbeeccbddd`c_b`cc chr7.fa 54159980 R 100 504 Y
>> HWI-ST538 160 7 1101 2566 1981 ACAGTG 1 ATTGAACTCANCTCCTTTTAGGTTGCCCTACTAACTGGACATTGTGGACACTAAATCATATTGGGGTGATGAATAATCAAATTTTCAATATCAAACAATG
>> abaceeeeggBQ`eghhiifhhiiigihifhihfhhihhiifhffdgffhifhihfhhhiihdgfgZd^bd^ade^abddc]`bc__bcdc_c_bb`abb chr10.fa 66474748 R 10G89 497 Y
>> HWI-ST538 160 7 1101 2943 1957 ACAGTG 1 TCCGAATANNNNNCGGTCAAAATAAAAGCTCAAGATGACATCAGTCCCATTTGTCCTAAGTCCTGGTGTTGTATGGATGGTAAGCAGCAGCCAATTATGG
>> [[[^^^^_BBBBBQQ_\_^___^_^_^^^^^^^^^^^^^^^^^^\^^^^^^^^^^^^^__\]^]^_]^^^^^_^_^^^^^W\]Z]]][[^[[[[W[\\\[ QC N
>> HWI-ST538 160 7 1101 2883 1967 ACAGTG 1 ATCAGACTCNNNCTGCACGTTCACACCATAAGTCTCATCAATGTTGTCATCCATATTCTGTATCTCCTTGTCGCCACCGTAGTCTGTGATCTTCTTGCCC
>> [[[__^^^^BBBR[T]]^^][]]^^[X][]]W^]^^^^X[Y\[YRY^[^^^^[]^^^^]^\Y]\Z^^[[ZU\\^BBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 3133 1960 ACAGTG 1 CTCTCAAANNNNCTACAGAGCCCAGGAAGATTTCAGGATGAAGAGCTTCCTCCTCTTCCTCACTATCATTCTTCTGGTTGTGATTCAGATACAAACAGGA
>> [[[^Z]_]BBBBSQ\_Z^_^_^^_^^^]_^^^^\^^^[Z]^^^^^^]^^^^^^]^]^^_]^ZZY^^_\]^\]^[]Z\^^Z\\]\\\\\\\]]]]\[[VQV QC N
>> HWI-ST538 160 7 1101 3023 1964 ACAGTG 1 GGCCACTCTNNNTTGTGCCTTCCACACCTTTTGGTGCAGATTGTTCAGTGGGGAGCTGCCTCATATTCTCTGACTGGCCAGAAAGGCTCTCGCTGGGGTT
>> [[[__^^^^BBBRQ_\_^^___^^^^_^^__^]^^^^^^^^^^^]\^^]^^^^^^^^^T]^^ZZ^\^]^^^]\\]]^BBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 3219 1966 ACAGTG 1 CAGATGTGTNNNAATGCTAGGTGTGGTTGGTTTATTCCTAGCGTCACTATTATCAGGCCTAGTTGGCTTGATGTAGAGAAGGCAATGATTTTTTTGATGT
>> [[[^_____BBBS[]___^__[_]_^\__^Z]^^^^^^^^^^^^^^^^^^^^^^]^^^^_[^^^^___^[^^]]]\\]\]]^^[[\\\\\\\\YXTW[\\ QC N
>> HWI-ST538 160 7 1101 3047 1993 ACAGTG 1 CTCTTGTGTCCGTTTTCTTGCTGTAGCGTTTTCTCCGTACCACTCACTGATACCTGTCTATCGACTACGTGGGATGGCAGAGGCACTGCCAGGCTCCACA
>> bbbeeedegggfghiiiihhiihifhiieggihffh[affgcgfebb]eefdfh[bgbcdfe_fW^\]aaZa`abZ^``a_GWWW^```^bS^`W^^BBB chr1.fa 154218045 F 100 36 Y
>> HWI-ST538 160 7 1101 3462 1958 ACAGTG 1 CCACTCGTNNNNCTTGCGATCATTGTGCTTAGGGTCTTCTGATACATCTGGGGGAACATCTGTTTTATCTGAACAAGGTCAATCTCACTTCGAGTGACCA
>> [[[^^^_\BBBBQQ[ZZ^_[Y_[_]_\W^^^^[^M[]]]]\]]^^^^]\^^^FZF\\]Z^^^X\^^^]]^]\Z\]ZOZXZZ[\\\\TZU\[ZTTW[\[\\ QC N
>> HWI-ST538 160 7 1101 3261 1972 ACAGTG 1 CCTGGATGTNNNCGTGGATCGGGTCTTGAACGTGGACTTGCCGCACCTTGTATCTCAAGCCCAGCGCCACACCCTTGCTGACTTTCTGAACACTACTGTC
>> BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 3390 1979 ACAGTG 1 TGCAGTTCTTNCCCGTGGCTCCTGAGCCAGCAATGTCGTCTGGGAAGCCATCATCAGCTCATTCAGCTGAGACGTGCTAGAGGTCTCCTCAGCTTGGAGC
>> bbbeeeeeggBRbfgfhihiiihhhhiiiiiehhichhgggfghbgedgdffhffdefff`bd]bdfedceecdZZ`_`bb_aL[bcc`b]`b]`b_`[` chr1.fa 187111962 F 10T89 497 Y
>> HWI-ST538 160 7 1101 3666 1959 ACAGTG 1 CCGAACACNNNNCCCGGATCTCATCTCACAGAATCGTGGGTACGTCTGAACCAGCTGGTTGCCCCCAAAAATTACCTGTTTCACGCCCCTGGTGCAGCCC
>> BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB QC N
>> HWI-ST538 160 7 1101 3667 1975 ACAGTG 1 AGAACACCGANGTCCACCTTCAAGTTAGGGGAAATGGCATATTCTTCGATCGGCTTGCTGATTTGGGAAATTTTATCCTTGACTGCTTCTGGAGCAGGAC
>> PZZTTP_BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB chr4.fa 99634649 R 10G3T10C4C19T1T13A10AG3T17 77 N
>> HWI-ST538 160 7 1101 3986 1969 ACAGTG 1 ATTAAATCTNNNCATGCTCTGAAAAAGGCTTTAGGTCACTCCAAGCTTGGCAGTTAACATTTGGCATGGACACTGGTAAAACCACAATAGAGAAAGAAGT
>> [[[^_____BBBRY__^^^__^^^^^_^^__^^^^^^^^^]^^^^^^^]^^^^\^^^^____]^____[^^[^^^^Z^Z\\\\^[^^^]]\[\\\\[[[R QC N
>> HWI-ST538 160 7 1101 4230 1977 ACAGTG 1 CGCCTCCGGGNCGTGGCACTCTGGGGCTCTGCCGTCGACATGGGCGCCGCC
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