[BioC] NOISeq-real input parameters
papori [guest]
guest at bioconductor.org
Mon Mar 19 10:38:00 CET 2012
Hi all,
i am using NOISeq with biological replicates.(i saw the Warning: NOISeq for biological replicates has not been tested yet. In case you use it, we will be very grateful to receive any feedback from you! , so, it will be a feedback in the end..)
I have 2 conditions with 3 replicates for each.
I normalized my data by using "tmm".
i execute NOISeq as:
myresults <- noiseq(mytmmdata_a,mytmmdata_na,repl = "bio" , k=0.5 , norm="tmm" , long=1000, q=0.8, nss=0)
My question is on the "q" parameter.
Usually significant is when p.v = 0.05.
What "q" value equal for that?
What is the "q" test? how calculate?
Thanks,
Papori
-- output of sessionInfo():
R version 2.14.0 (2011-10-31)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 LC_PAPER=C LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] edgeR_2.4.3 limma_3.10.3
loaded via a namespace (and not attached):
[1] annotate_1.32.2 AnnotationDbi_1.16.19 Biobase_2.14.0 DBI_0.2-5 DESeq_1.6.1
[6] genefilter_1.36.0 geneplotter_1.32.1 grid_2.14.0 IRanges_1.12.6 RColorBrewer_1.0-5
[11] RSQLite_0.11.1 splines_2.14.0 survival_2.36-12 tools_2.14.0 xtable_1.7-0
--
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