[BioC] Error by using gpr data with marray
Sandra Heise [guest]
guest at bioconductor.org
Fri Mar 9 11:19:24 CET 2012
hi,
I am just a beginner in using R and bioconductor. I have a microarray experiment from the arrayexpress database.
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-109
There are given the target txt and row data (gpr). Now i want used
bioconductor to generate a nomalized intensity matrix for all knockout experiments. I thought i have to use limma package and tried to read the data with marray function read.GenePix but there is still an error. Then I tried to read the info txt but there is still an error too. Maybe i just chose the wrong package for my dataset. Unfortunately I don't have a clue how i can generate the normalized datamatrix but the autors of the dataset wrote thez generate the log_fold_change matrix with Imfit limma function. So i thought there is a possibility for me.
please help
-- output of sessionInfo():
library(limma)
library(marray)
read.GenePix("3088.gpr")
> Error in if (skip > 0L) readLines(file, skip) :
> missing value where TRUE/FALSE needed
a.target <- read.marrayInfo("E-MTAB-109.sdrf.txt")
> Error in names(x) <- value :
> 'names' attribute [30] must be the same length as the vector [7]
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