[BioC] using Limma to read 2-channel dye-swap in Agilent scanner
Neta
netazuck at gmail.com
Thu Jun 14 01:47:37 CEST 2012
Hello,
I am using Limma (version 3.12.0) to read 2-channel dye-swap files
from an Agilent image analysis scanner, where each sample has 2
files - one for cy3 and one for cy5.
However, the only reference to such a 2-file input in the limma user
guide is to "ImaGene".
When I try to read the files and the target file using the following
commands:
RG = read.maimages(file_names, ...)
targets = readTargets("targets.txt")
files = targets[,c("FileNameCy3","FileNameCy5")];
RG = read.maimages(files,source="imagene");
I get the following error message:
Error in read.imagene(files = files, path = path, ext = ext, names = names,
: Can't find Field Dimensions in ImaGene header
In addition: Warning message:
In readImaGeneHeader(fullname) : End of file encountered before
End Header
When I try to use source="Agilent" instead of "imagene", the command
doesn't seem to understand that I have 2 file names, and gives the following
error message:
Error in read.maimages(files, source = "agilent") :
targets frame doesn't contain FileName column
I tried every single option for "source" that was listed in the help of
"read.maimages" but it seems like "imagene" is the only one that is
able to digest the two headers for the file names.
I am stuck and would appreciate any help.
Thank you,
Neta.
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