[Bioc-sig-seq] DESeq

[Nicolas Delhomme]

Hi Bogdan,,

As specified in the vignette, you should use raw tag counts and never RPKM. DESeq will perform the library size adjustment.

Cheers,

Nico

---------------------------------------------------------------
Nicolas Delhomme

High Throughput Functional Genomics Center

European Molecular Biology Laboratory

Tel: +49 6221 387 8310
Email: nicolas.delhomme at embl.de
Meyerhofstrasse 1 - Postfach 10.2209
69102 Heidelberg, Germany
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On 26 Jul 2011, at 22:16, Bogdan Tanasa wrote:

> Dear all,
> 
> regarding DESeq package: would it be more appropriate to use raw tag counts
> or RPKM values ? thanks,
> 
> bogdan
> 
> 	[[alternative HTML version deleted]]
> 
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