[Bioc-sig-seq] qa error in ShortRead
Kunbin Qu
KQu at genomichealth.com
Wed Sep 15 06:27:10 CEST 2010
Hi,
I received the following error when I tried to read some fastq in and
generate QA report. Could anybody help me? Thanks.
-Kunbin
[srp101]more tmp2
@SEQUENCER01_0007:1:1:1066:13331#0/1
CAGCTANCTGCGTTCTTCATCGACGCACGAGCCGAGTATTAATGTGAATTG
+SEQUENCER01_0007:1:1:1066:13331#0/1
fdfffcBccccccccfffffffeffffffcfffffedcfcffffddedfdd
@SEQUENCER01_0007:1:1:1066:8699#0/1
CTTACCNCCCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCAC
+SEQUENCER01_0007:1:1:1066:8699#0/1
cc^ccaBV]]ba_^Zc^cbaa`c`cL^```cb\ccc^aaaaa^aabba`bb
@SEQUENCER01_0007:1:1:1066:20185#0/1
GCCACANACACCTGATTATCAACAGCCCGGCTTCGCTGAAGTAACTCCCAA
+SEQUENCER01_0007:1:1:1066:20185#0/1
fffffbBbbbcccccfffffefffffffffeffffffffefffffdefffd
[srp101]
> s6<-readFastq("./", "tmp2")
> q6<-qa(s6)
Error: UserArgumentMismatch
'lane' must be 'character(1)'
> sessionInfo()
R version 2.11.0 (2010-04-22)
x86_64-unknown-linux-gnu
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] ShortRead_1.6.2 Rsamtools_1.0.1 lattice_0.18-8
[4] Biostrings_2.16.7 GenomicRanges_1.0.1 IRanges_1.6.8
loaded via a namespace (and not attached):
[1] Biobase_2.8.0 grid_2.11.0 hwriter_1.2
>
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