[BioC] problem with EdgeR script
Jim Tepperman [guest]
guest at bioconductor.org
Wed Mar 26 01:13:11 CET 2014
I am trying to run an EdgeR script that I have been using for several years. I am getting an error message early on in the script and cannot figure out what is wrong. Please advise.
-- output of sessionInfo():
The downloaded binary packages are in
/var/folders/Fx/FxdWG0DyE3eRfY72Zl3JxU+++TI/-Tmp-//RtmpoQbYDm/downloaded_packages
> library(limma)
> library (edgeR)
> mydata<-read.delim(file="wt_vs_pifq.txt",head=TRUE)
> head (mydata)
locus WT1A WT1B WT2A WT2B WT3A WT3B PQ1A PQ1B PQ2A PQ2B PQ3A PQ3B
1 AT1G01010 88 117 87 105 76 69 54 24 58 52 68 57
2 AT1G01020 36 156 49 49 89 46 53 34 70 54 63 66
3 AT1G01030 8 15 12 8 8 11 6 6 7 9 15 5
4 AT1G01040 42 120 94 47 85 63 27 15 63 34 49 54
5 AT1G01046 0 0 0 0 0 0 0 0 0 0 0 0
6 AT1G01050 461 876 424 606 452 369 358 168 348 446 299 285
> raw.data<- mydata
> names(raw.data)
[1] "locus" "WT1A" "WT1B" "WT2A" "WT2B" "WT3A" "WT3B" "PQ1A" "PQ1B" "PQ2A" "PQ2B" "PQ3A" "PQ3B"
> d<-raw.data[,2:13]
> rownames(d)<-raw.data[,1]
Error in `row.names<-.data.frame`(`*tmp*`, value = value) :
duplicate 'row.names' are not allowed
In addition: Warning message:
non-unique value when setting 'row.names': ââ
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