[BioC] problem with EdgeR script

Jim Tepperman [guest] guest at bioconductor.org
Wed Mar 26 01:13:11 CET 2014


I am trying to run an EdgeR script that I have been using for several years.  I am getting an error message early on in the script and cannot figure out what is wrong.  Please advise.

 -- output of sessionInfo(): 


The downloaded binary packages are in
	/var/folders/Fx/FxdWG0DyE3eRfY72Zl3JxU+++TI/-Tmp-//RtmpoQbYDm/downloaded_packages
> library(limma)
> library (edgeR)
> mydata<-read.delim(file="wt_vs_pifq.txt",head=TRUE)
> head (mydata)
      locus WT1A WT1B WT2A WT2B WT3A WT3B PQ1A PQ1B PQ2A PQ2B PQ3A PQ3B
1 AT1G01010   88  117   87  105   76   69   54   24   58   52   68   57
2 AT1G01020   36  156   49   49   89   46   53   34   70   54   63   66
3 AT1G01030    8   15   12    8    8   11    6    6    7    9   15    5
4 AT1G01040   42  120   94   47   85   63   27   15   63   34   49   54
5 AT1G01046    0    0    0    0    0    0    0    0    0    0    0    0
6 AT1G01050  461  876  424  606  452  369  358  168  348  446  299  285
> raw.data<- mydata
> names(raw.data)
 [1] "locus" "WT1A"  "WT1B"  "WT2A"  "WT2B"  "WT3A"  "WT3B"  "PQ1A"  "PQ1B"  "PQ2A"  "PQ2B"  "PQ3A"  "PQ3B" 
> d<-raw.data[,2:13]
> rownames(d)<-raw.data[,1]
Error in `row.names<-.data.frame`(`*tmp*`, value = value) : 
  duplicate 'row.names' are not allowed
In addition: Warning message:
non-unique value when setting 'row.names': ‘’ 

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