[BioC] edgeR time series analysis question
Audra Andrew [guest]
guest at bioconductor.org
Sat Mar 8 04:39:49 CET 2014
Hi,
I have RNAseq data in replicates for 6 time points (0h, 6h, 12h, 24h, 96h, and 10 days). I want to do time course analysis of this data using GLM. Experimenting, I used a model matrix with an intercept term and the section on the ANOVA-like test for any differences. My question: Is the list of genes output based on contrasts between all the time points or just between each with the intercept(0h)? I hope this makes sense. Thanks so much!
Best,
Audra
-- output of sessionInfo():
> sessionInfo()
R version 3.0.2 (2013-09-25)
Platform: x86_64-apple-darwin10.8.0 (64-bit)
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] tcltk parallel stats graphics grDevices utils
[7] datasets methods base
other attached packages:
[1] maSigPro_1.34.0 DynDoc_1.40.0 widgetTools_1.40.0
[4] MASS_7.3-29 Biobase_2.22.0 BiocGenerics_0.8.0
[7] edgeR_3.4.2 limma_3.18.9
loaded via a namespace (and not attached):
[1] Mfuzz_2.20.0 tkWidgets_1.40.0 tools_3.0.2
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