[BioC] filterBam using rname
rcaloger
raffaele.calogero at gmail.com
Thu Oct 18 15:57:34 CEST 2012
I have the necessity to filter a Bam file, keeping only reads
associated to a subset of references.
I wanted to do it with filterBam (Rsamtools)
but I could not manage to find a way of doing it.
If I use the code:
filterBam("onlymapped.bam", "filtered.bam",
param=ScanBamParam(which=my.rnames, what=scanBamWhat("rname")))
I get:
Error in function (classes, fdef, mtable) :
unable to find an inherited method for function "ScanBamParam", for
signature "character"
checking the help it seems to me that ScanBamParam(which=) only support
GRanges, RangesList, RangedData objects.
There is any other way to filter a bam file on the basis of the rname?
Cheers
Raffaele
> sessionInfo()
R version 2.15.1 (2012-06-22)
Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit)
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] ShortRead_1.14.4 latticeExtra_0.6-24 RColorBrewer_1.0-5
lattice_0.20-10 Rsamtools_1.8.6
[6] Biostrings_2.24.1 GenomicRanges_1.8.13 IRanges_1.14.4
Biobase_2.16.0 BiocGenerics_0.2.0
loaded via a namespace (and not attached):
[1] bitops_1.0-4.1 grid_2.15.1 hwriter_1.3 stats4_2.15.1
tools_2.15.1 zlibbioc_1.2.0
>
--
----------------------------------------
Prof. Raffaele A. Calogero
Bioinformatics and Genomics Unit
MBC Centro di Biotecnologie Molecolari
Via Nizza 52, Torino 10126
tel. ++39 0116706457
Fax ++39 0112366457
Mobile ++39 3333827080
email: raffaele.calogero at unito.it
raffaele[dot]calogero[at]gmail[dot]com
www: http://www.bioinformatica.unito.it
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