[BioC] NOISeq initialization problem
sara wilson [guest]
guest at bioconductor.org
Thu Oct 11 10:32:08 CEST 2012
Hi and good day,
I am totally new to R so sorry is my question is so naive.
I am just following the NOISeq tutorial with my own data set.Below is what I have in order to make the readData:
my data:
> head(counts)
V2 V3 V4 V5 V6 V7 V8 V9
1/2-SBSRNA4 3 5 4 4 2 3 1 1
A1BG 200 93 246 102 86 46 58 85
A1BG-AS1 24 28 16 32 17 10 19 14
A1CF 1 1 1 2 1 0 0 1
A2LD1 100 71 98 97 59 128 88 114
A2M 5 5 23 1 5 6 10 5
> class(counts)
[1] "data.frame"
my factors :
> factors
sex
1 Female
2 Female
3 Female
4 Female
5 Male
6 Male
7 Male
8 Male
and my length :
> head(mylength)
1/2-SBSRNA4 A1BG A1BG-AS1 A1CF A2LD1 A2M
23 916 160 7 755 60
> class(mylength)
[1] "integer"
when I run the command readData, without the length property it produces the object but with the length property, there is an error. so there should be problem with that but I do not know what is it.
Many thanks in advance.
-- output of sessionInfo():
> noy1 <- readData(data=counts, factors=factors, length= mylength)
> head(noy1)
Error in value[[3L]](cond) : invalid 'row.names' length
AnnotatedDataFrame 'initialize' could not update varMetadata:
perhaps pData and varMetadata are inconsistent?
and then without length:
> noy1 <- readData(data=counts, factors=factors)
> head(noy1)
ExpressionSet (storageMode: lockedEnvironment)
assayData: 1 features, 8 samples
element names: exprs
protocolData: none
phenoData
sampleNames: V2 V3 ... V9 (8 total)
varLabels: sex
varMetadata: labelDescription
featureData: none
experimentData: use 'experimentData(object)
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