[BioC] shan

wang peter wng.peter at gmail.com
Wed Oct 3 15:48:25 CEST 2012


dear steve:
you are right,

1.  51 bp lib is too short for assembly

2.  we have two condition of samples
(DLCK1-51bp	DLCK2-51bp	DLCK3-51bp) vs (LCK5-51bp	LCK6-51bp	LCK7-51bp)

then we sequencing the same samples by 100bp

(DLCK1	DLCK2	DLCK3) vs (LCK5	LCK6	LCK7)

3 i want to find DE genes between DLCK vs LCK

BUT i donot want to combine 51 bp with 100 bp data, which removing the
replicate variation
(DLCK1+DCLK1-51bp 	DLCK2....	DLCK3.....) vs ....

shan





-- 
shan gao
Room 231(Dr.Fei lab)
Boyce Thompson Institute
Cornell University
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