[BioC] vsn2 dimension of the expression level matrix

[Sam Wong]

Hi,

I am using vsn2 to normalize an expression. Here are my syntax:

controls<-vsn2(rawdata[idx.control,])
genes.vsn2<-predict(controls,newdata=rawdata[-idx.control,])

I got the following error message:

Error in .local(x, reference, strata, ...) : 
  One or more of the strata contain less than 42 elements.
Please reduce the number of strata so that there is enough in each stratum.

The controls matrix only has 33 rows.  Does vsn2 require the number of rows for the data matrix to be a minimum of 42?


Thanks,

Sam Wong