[BioC] error while using genefilter's nsFilter: could not find function "hgug4112a.db_dbconn"
James W. MacDonald
jmacdon at med.umich.edu
Fri Oct 30 14:06:37 CET 2009
Hi Massimo,
Massimo Pinto wrote:
> Greetings all,
>
> I have finally come across to genefilter's nsFIlter(); it looks like
> it's going to do quite a lot for me in just one call. I called it as
> follows:
>
>> esetGSEA <- nsFilter(esetPROC, require.entrez=TRUE, require.GOBP=TRUE, remove.dupEntrez=TRUE, var.func=IQR, var.cutoff=0.5)
> Error in do.call(paste(chip, "_dbconn", sep = ""), list()) :
> could not find function "hgug4112a.db_dbconn"
This could probably use a better error message. The problem is that you
don't have the annotation package for this chip available, so you cannot
filter based on things like Entrez Gene, GO BP, etc.
I assume this is a custom chip, so you will need to get the annotation
file from Affy and build an annotation package yourself using SQLForge.
See the SQLForge vignette for more information:
http://www.bioconductor.org/packages/release/bioc/vignettes/AnnotationDbi/inst/doc/SQLForge.pdf
Best,
Jim
>
>> annotation(esetPROC)
> [1] "hgug4112a.db"
>
> here's some info on my session:
>
>> sessionInfo()
> R version 2.10.0 (2009-10-26)
> x86_64-apple-darwin9.8.0
>
> locale:
> [1] C
>
> attached base packages:
> [1] grid tcltk stats graphics grDevices utils
> datasets methods base
>
> other attached packages:
> [1] affy_1.24.0 gplots_2.7.3 caTools_1.10
> bitops_1.0-4.1 gdata_2.6.1 gtools_2.6.1
> [7] hgug4112a.db_2.3.5 org.Hs.eg.db_2.3.6 RSQLite_0.7-3
> DBI_0.2-4 Agi4x44PreProcess_1.5.1
> genefilter_1.28.0
> [13] annotate_1.24.0 AnnotationDbi_1.7.20 limma_3.2.1
> Biobase_2.6.0
>
> loaded via a namespace (and not attached):
> [1] affyio_1.13.5 preprocessCore_1.7.9 splines_2.10.0
> survival_2.35-7 tools_2.10.0 xtable_1.5-5
>
> Thanks a lot,
> Yours
> Massimo
>
> Massimo Pinto
> Post Doctoral Research Fellow
> Enrico Fermi Centre and Italian Public Health Research Institute (ISS), Rome
> http://claimid.com/massimopinto
>
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--
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
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