[BioC] ArrayQualityMetrics: Problem with labels in plots. A bug??

Audrey Kauffmann audrey at ebi.ac.uk
Fri Oct 30 10:51:39 CET 2009


Dear Jeremy,

Sorry I did not think about this, I think it takes a couple of days 
between the moment the developer modifies the version of the package and 
the moment you can actually install it using biocLite. You should be 
able to install version 2.4.1 after the week-end.

Best wishes,
Audrey

jeremy wilson wrote:
> Dear Audrey,
>
> thanks for your reply. I tried re-installing the package from bioc. It 
> gave the same results. I later installed 2.5 version of the software. 
> This also gave the same plots. There is still the same problem with 
> ordering of colors.
> I see you asked me to use version 2.4.1 but I could not find it in the 
> release or development version. Am I looking at the wrong places? 
> Please let me know and thanks for your time...
>
> Thank you
> Lohith
>
> P.S: I know this might be just a simple bug in the program and I 
> wanted to see if I can successfully tweak the R script for the 
> software but I did not find the R script in both the release and 
> development versions..It would be great to see the code..
>
>
> On Thu, Oct 29, 2009 at 2:41 AM, Audrey Kauffmann <audrey at ebi.ac.uk 
> <mailto:audrey at ebi.ac.uk>> wrote:
>
>     Dear Jeremy,
>
>     Thank you for reporting this. You were right there was a problem
>     in the code for the ordering of the colours.
>     I have now corrected it, so arrayQualityMetrics version 2.4.1
>     should be working fine.
>     Please let me know if you keep experiencing problems.
>
>     Best wishes,
>     Audrey
>
>     jeremy wilson wrote:
>
>         Dear BioConductors,
>
>         I am using arrayQualityMetrics software package to do the QA.
>         I very much
>         like all the QA plots it produces including the latest PCA
>         plot in one
>         report. I am using the latest version of R(2.10) and BioC
>         (2.5). I am facing
>         a problem with inappropriate labeling of covariates. Please
>         see the attached
>         plots.
>         I have total of 69 samples. I asked the software to label the
>         plots based on
>         a covariate, the summary of which is as below:
>
>         Notation    total number of samples in order
>         V                         23
>         G                         38
>         N                          8
>
>         The problem is that it does not label the graphs correctly in
>         the plots!! In
>         the plots, the first set of samples correspond to V, the next
>         38 samples for
>         G and the last 8 for N. In the boxplot for example, the G was
>         labelled RED
>         and this should correspond to 38 samples but in the plot, the
>         boxplots
>         corresponding to these 38 samples were colored GREEN. The ones
>         that were
>         labeled RED correspond to 8 'N' samples which should be
>         represented in blue
>         according to the label at the top of plot.  Likewise for the
>         other two. I
>         think it can be better understood at the first glance of the
>         plots.
>
>         The phenotype of the samples was given correctly.
>         Following is the code I used for generating the aqm report.
>
>         library(arrayQualityMetrics)
>         arrayQualityMetrics(rat.ab, do.logtransform=TRUE, outdir="QA",
>         intgroup="Material", grouprep=TRUE)
>
>         I have attached only a few plots. I can produce all of them if
>         it helps...
>         It might take some time for the post to reach the mailing list
>         as the
>         earlier one I posted with a few more plots was held for
>         moderator's approval
>         as the size of the message body is over the limit!
>
>         Please let me know what is wrong.
>         Thanks in advance!
>          
>         ------------------------------------------------------------------------
>
>
>         ------------------------------------------------------------------------
>
>         ------------------------------------------------------------------------
>
>
>
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>
>     -- 
>     Audrey Kauffmann
>     EMBL - EBI
>     Cambridge UK
>     +44 (0) 1223 492 631 http://www.ebi.ac.uk/~audrey
>     <http://www.ebi.ac.uk/%7Eaudrey>
>
>

-- 
Audrey Kauffmann
EMBL - EBI
Cambridge UK
+44 (0) 1223 492 631 
http://www.ebi.ac.uk/~audrey



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