[BioC] Affymetrix Mouse Promoter array / pdInfoBuilder
Hans-Ulrich Klein
h.klein at uni-muenster.de
Mon Oct 12 16:09:43 CEST 2009
Hi All,
I have ChIP-chip data based on the Affymetrix Mouse Promoter platform. I
built a "pd.mm.prompr.v02" package with the pdInfoBuilder functions and
read in the CEL files using the oligo package.
I get stuck with the genome coordinates of the probes provided by my
"pd.mm.prompr.v02" package. The pdInfoBuilder takes a "bpmap" file that
can be downloaded from affymetrix.com. The newest file has coordinates
for the "NCBI36" mouse assembly and thus is outdated. Ensembl (and so
biomaRt that I want to use for genome annotation) uses NCBI m37 assembly.
Is there a convenient way to integrate newer coordinates or sequences
provided as text files into the annotation package created by
pdInfoBuilder? Working with a large data.frame would perhaps be to slow.
BTW, does anyone know where I can download the probes' sequences? I have
not found them on affymetrix.com yet.
Best,
Hans-Ulrich
--
Hans-Ulrich Klein
Department of Medical Informatics and Biomathematics
University of Münster
Domagkstrasse 9
48149 Münster, Germany
Tel.: +49 (0)251 83-58405
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