[BioC] annotation for illumina mouse-6 arrays? & GOstats
Yupu Liang
liang at cbio.mskcc.org
Fri Oct 2 01:56:26 CEST 2009
Hi,
Sorry for the long email,
I have two questions towards using GOstats for illumina mouse-6 data:
1-- I couldn't find the annotation package for illumina mouse-6 arrays
under the downloads link,
Is there a way for me to generate it myself? I do have the annotation
information for the platform?
2--I tried to generated the entrez list for all the genes from the
chip through the following code -- ulist is list of gene symbol from
the chip
x <- org.Mm.egSYMBOL2EG
mapped_genes <- mappedkeys(x)
xx <- as.list(x[mapped_genes])
ulist.entrez<-xx[ulist]
ulist.e <- unlist(glist.entrez,use.names=F)
The problem of my code is there was original 20183 symbols, after
remove the duplications(multiple probes for one gene), I got 14430
genes.
But only 244 of them have entrez ids -- which makes no GO term
significant.
> glist.entrez[1:5]
$`1110012J17Rik`
[1] "68617"
$<NA>
NULL
$`1110032E23Rik`
[1] "68659"
$<NA>
NULL
$<NA>
NULL
I am not sure what I did wrong? or how to get around this problem?
Thanks,
Yupu
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