[Bioc-sig-seq] a question

Joseph Scandura jms2003 at med.cornell.edu
Fri Sep 2 20:00:13 CEST 2011


Phosphate


On Sep 2, 2011, at 10:34 AM, wang peter wrote:

> i read the illumina premers document
> ther is a "P" letter in the front of adapters1
> what does it mean?
> 
> thx
> shan gao
> 
> Genomic DNA/ChIP-Seq oligonucleotide sequences
> Adapters1
> 5' P-GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
> 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
> PCR Primers1
> 5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
> 5' CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
> Genomic DNA Sequencing Primer
> 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
> Paired End DNA oligonucleotide sequences
> PE Adapters1
> 5' P-GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
> 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
> PE PCR Primers1
> 5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
> 5' CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
> PE Sequencing Primer
> 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
> 5' CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
> 
> 	[[alternative HTML version deleted]]
> 
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