[Bioc-sig-seq] pileup() for small RNA sequencing

[Wu, Xiwei]

Dear all,

I have been working on a small RNA sequencing data set. After adapter
trimming, the length of each tag is different, ranging from 16 to 40.
While I am using pileup function in SHortRead package to calculate the
coverage, it only allows a uniform length of tags, which will generate
inaccurate coverage. Is it possible to set different tag length, or any
other functions allowing this in other packages? Thanks in advance.

Xiwei Wu
City of Hope


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