[Bioc-sig-seq] Scatter Plot Matrix

[Clive G. Brown]

4 nucs - 2 lasers.


On 07/11/2008 14:47, "James W. MacDonald" <jmacdon at med.umich.edu> wrote:

> Martin Morgan wrote:
>> As I understand it, there are two (not four, as one might think)
>> florescent nucleotides ('dATP', 'dCTP'), and each is measured on two
>> different wavelengths ('red', 'green'). This is from here
>> 
>> http://dx.doi.org/10.1016/S0003-2697(03)00291-4
> 
> According to this demo on Illumina's site, they use four labeled
> nucleotides.
> 
> http://www.illumina.com/media.ilmn?Title=Sequencing-By-Synthesis%20Demo&Cap=&I
> mg=spacer.gif&PageName=illumina%20sequencing%20technology&PageURL=203&Media=1
> 
> But as you note in the scatterplot (and as well in this paper by Naef
> http://www.biomedcentral.com/1471-2105/9/431), the A and C channels and
> T and G channels are highly correlated.
> 
> Best,
> 
> Jim
> 
> 

--
Clive G. Brown
Director, Bioinformatics & I.T.
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